Wild bees (Apoidea: Anthophila) of South-West France: more than 10 years of inventories in mosaic landscapes of ZA-PYGAR

Mélodie Ollivier1, Justine Rivers-Moore2, Magalie Pichon3, Emilie Andrieu3, Romain Carrié3, Rémi Rudelle4, Jean-Pierre Sarthou5, Annie Ouin1, Annie Ouin6

1 UMR DYNAFOR INP-ENSAT, LTSER Zone Atelier “PYRÉNÉES GARONNE”, 31320, Auzeville-Tolosane, France; 2 Facultad de Agronomía y Sistemas Naturales, Pontificia Universidad Católica de Chile, Santiago, Chile; 3 UMR DYNAFOR INRAE, LTSER Zone Atelier “PYRÉNÉES GARONNE”, 31320, Auzeville-Tolosane, France; 4 Rudélide Expertise Muséologie, Rieupeyroux, France; 5 Centre de Recherche sur la Biodiversité et l'Environnement (CRBE), Université de Toulouse, CNRS, IRD, Toulouse INP, Université Toulouse 3 – Paul Sabatier (UT3), Toulouse, France; 6 UMR DYNAFOR INP-ENSAT, Auzeville, France

Corresponding author(s): Mélodie Ollivier (melodie.ollivier@toulouse-inp.fr)Justine Rivers-Moore (justine.riversmoore@protonmail.com)Magalie Pichon (magalie.pichon@inrae.fr)Emilie Andrieu (emilie.andrieu@inrae.fr)Romain Carrié (romain.carrie@cec.lu.se)Rémi Rudelle (remirud@gmail.com)Jean-Pierre Sarthou (jean-pierre.sarthou@toulouse-inp.fr)Annie Ouin (annie.ouin@toulouse-inp.fr)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Ollivier M, Rivers-Moore J, Pichon M, Andrieu E, Carrié R, Rudelle R, Sarthou J, Ouin A (2024): Wild bees (Apoidea: Anthophila) of South-West France: more than 10 years of inventories in mosaic landscapes of ZA-PYGAR. v1.4. Institut national de recherche pour l’agriculture, l’alimentation et l’environnement (INRAE). Dataset/Occurrence. http://dynids.toulouse.inra.fr:8180/ipt/resource?r=baseabeilles&v=1.4

Abstract

We provide here data for the 24,329 wild bee specimens (Apoidae: Anthophila) held in the collection of DYNAFOR Lab (UMR 1201 INRAE, INP-ENSAT, EI PURPAN), located at INP-ENSAT (Toulouse, France). These data have been published as a datapaper in Biodiversity Data Journal (Ollivier et al, 2024).

Keywords: Occurrence, Observation

Project details

Project title: Wild bees (Apoidea) of South-West France: more than 10 years of inventories in mosaic landscapes of ZA-PYGAR
Personnel: Mélodie Ollivier, Justine  Rivers-Moore, Magalie Pichon, Emilie Andrieu, Romain Carrié, Rémi Rudelle, Jean-Pierre Sarthou, Annie Ouin
Funding: Bilisse (French Ministry of Ecology and Sustainable Development - GIP Ecofor), Farmland (ANR-11-EBID-0004), ForestBees (Défi chercheur Inrae 2021), BioBio (EU FP7 project KBBE- 227161), Sebioref (PSDR 4 Occitanie)
Study area descriptions/descriptor: The LTSER ZA PYGAR site took place in South Western of France in the foothills of the Pyrénées mountains. It is characterised by mixed crops and extensive livestock farming systems. These agronomic features and topographic constraints have designed highly heterogeneous landscapes where wooded elements (hedgerows, scattered trees, small forests) are embedded in a mosaic of annual crops and permanent or temporary meadows (Ouin et al. 2022).  According to the scientific question investigated, sample sites were chosen along gradients of landscape heterogeneity (composition and configuration) and production system (conventional to organic farming).
Design description: See sampling methods. 

Data published through GBIF: http://dynids.toulouse.inra.fr:8180/ipt/resource?r=baseabeilles

Taxonomic coverage

General taxonomic coverage description: The dataset contains at least 270 species of the families Andrenidae (2,958 specimens), Apidae (2,805 specimens), Colletidae (167 specimens), Halictidae (18,007 specimens), Megachilidae (377 specimens) and Melittidae (15 specimens). Thirty-three genera have been identified, the most represented being Lasioglossum , Andrena and Halictus. Of the 275 taxon (comprising 270 species and 5 species complexes), 29 taxon are represented by more than 100 specimens, and 134 taxon are represented by 1 to 5 individuals, 56 being singletons. 
Taxonomic ranks
Family: Andrenidae , Apidae , Colletidae , Halictidae , Megachilidae , Melittidae 
Genus: Seladonia Robertson, 1918, Hylaeus Fabricius, 1793, Lasioglossum Curtis, 1833, Sphecodes Latreille, 1804, Eucera Scopoli, 1770, Andrena Fabricius, 1775, Nomada Scopoli, 1770, Osmia Panzer, 1806
Species: Seladonia tumulorum (Linnaeus, 1758), Lasioglossum pauxillum (Schenck, 1853), Lasioglossum politum (Schenck, 1853), Andrena variabilis Smith, 1853, Bombus lapidarius (Linnaeus, 1758), Halictus scabiosae (Rossi, 1790), Lasioglossum puncticolle (Morawitz, 1872), Lasioglossum villosulum (Kirby, 1802), Bombus pratorum (Linnaeus, 1761), Lasioglossum malachurum (Kirby, 1802), Melitta nigricans Alfken, 1905, Bombus terrestris (Linnaeus, 1758), Lasioglossum glabriusculum (Morawitz, 1872), Eucera nigrifacies Lepeletier, 1841, Halictus compressus (Walckenaer, 1802), Bombus Latreille, 1802, Halictus Latreille, 1804, Andrena labialis (Kirby, 1802), Lithurgus cornutus (Fabricius, 1787), Andrena flavipes Panzer, 1799, Andrena impunctata Perez, 1895, Seladonia seladonia (Fabricius, 1794), Lasioglossum interruptum (Panzer, 1798), Halictus simplex Bluthgen, 1923, Halictus maculatus Smith, 1848, Andrena wilkella (Kirby, 1802), Andrena ventricosa Dours, 1873, Lasioglossum corvinum (Morawitz, 1877), Bombus sylvarum (Linnaeus, 1761), Nomada kohli Schmiedeknecht, 1882, Nomada distinguenda Morawitz, 1873, Heriades truncorum (Linnaeus, 1758), Lasioglossum leucozonium (Schrank, 1781), Seladonia subaurata (Rossi, 1792), Xylocopa violacea (Linnaeus, 1758), Hylaeus clypearis (Schenck, 1853), Sphecodes longulus Hagens, 1882, Sphecodes puncticeps Thomson, 1870, Seladonia smaragdula (Vachal, 1895), Hylaeus variegatus (Fabricius, 1798), Hylaeus brevicornis Nylander, 1852, Hylaeus euryscapus Forster, 1871, Bombus pascuorum (Scopoli, 1763), Lasioglossum albipes (Fabricius, 1781), Bombus hortorum (Linnaeus, 1761), Lasioglossum zonulum (Smith, 1848), Nomada stigma Fabricius, 1804, Andrena decipiens Schenck, 1861, Eucera nigrescens Perez, 1880, Halictus quadricinctus (Fabricius, 1777), Dasypoda hirtipes (Fabricius, 1793), Eucera taurica Morawitz, 1871, Lasioglossum discus (Smith, 1853), Eucera longicornis (Linnaeus, 1758), Lasioglossum lativentre (Schenck, 1853), Eucera clypeata Erichson in Waltl, 1835, Lithurgus chrysurus Fonscolombe, 1834, Hylaeus difformis (Eversmann, 1852), Osmia gallarum Spinola, 1807, Lasioglossum marginatum (Brulle, 1832), Lasioglossum nigripes (Lepeletier, 1841), Heriades crenulata Nylander, 1856, Ceratina cyanea (Kirby, 1802), Lasioglossum punctatissimum (Schenck, 1853), Andrena ranunculi Schmiedeknecht, 1884, Coelioxys afer Lepeletier, 1841, Lasioglossum morio (Fabricius, 1793), Halictus langobardicus Bluthgen, 1944, Osmia rufohirta Latreille, 1811, Andrena ovata Schenck, 1853, Stelis punctulatissima (Kirby, 1802), Hylaeus hyalinatus Smith, 1842, Lasioglossum laticeps (Schenck, 1869), Osmia caerulescens (Linnaeus, 1758), Andrena aeneiventris Morawitz, 1872, Megachile burdigalensis Benoist, 1940, Anthidium oblongatum (Illiger, 1806), Ceratina cucurbitina (Rossi, 1792), Hoplitis tridentata (Dufour & Perris, 1840), Andrena chrysosceles (Kirby, 1802), Hoplitis leucomelana (Kirby, 1802), Nomada fucata Panzer, 1798, Rophites algirus Perez, 1895, Anthidium manicatum (Linnaeus, 1758), Melitta leporina (Panzer, 1799), Tetralonia alticincta (Lepeletier, 1841), Andrena agilissima (Scopoli, 1770), Hylaeus gibbus Saunders, 1850, Andrena bicolor Fabricius, 1775, Andrena florea Fabricius, 1793, Anthophora pubescens (Fabricius, 1781), Hylaeus tyrolensis Forster, 1871, Seladonia submediterranea Pauly, 2015, Seladonia vestita (Lepeletier, 1841), Megachile rotundata (Fabricius, 1787), Megachile willughbiella (Kirby, 1802), Megachile maritima (Kirby, 1802), Ceratina chalybea Chevrier, 1872, Megachile apicalis Spinola, 1808, Xylocopa valga Gerstacker, 1872, Osmia spinulosa (Kirby, 1802), Sphecodes rufiventris (Panzer, 1797), Xylocopa iris (Christ, 1791), Hylaeus gredleri Forster, 1871, Nomada basalis Herrich-Schaffer, 1839, Hylaeus punctulatissimus Smith, 1843, Amegilla albigena (Lepeletier, 1841), Lasioglossum semilucens (Alfken, 1914), Trachusa interrupta (Fabricius, 1781), Sphecodes ferruginatus Hagens, 1882, Sphecodes monilicornis (Kirby, 1802), Pseudoanthidium scapulare (Latreille, 1809), Bombus ruderarius (Muller, 1776), Anthidiellum strigatum (Panzer, 1802), Bombus humilis Illiger, 1806, Hylaeus confusus Nylander, 1852, Hylaeus nigritus (Fabricius, 1798), Ceratina dallatorreana Friese, 1896, Andrena alfkenella Perkins, 1914, Andrena minutula (Kirby, 1802), Sphecodes ephippius (Linnaeus, 1767), Lasioglossum calceatum (Scopoli, 1763), Bombus confusus Schenck, 1861, Bombus lucorum (Linnaeus, 1761), Nomada flavoguttata (Kirby, 1802), Andrena propinqua Schenck, 1853, Hylaeus communis Nylander, 1852, Lasioglossum fulvicorne (Kirby, 1802), Andrena afzeliella (Kirby, 1802), Megachile ericetorum Lepeletier, 1841, Bombus vestalis (Geoffroy in Fourcroy, 1785), Colletes daviesanus Smith, 1846, Megachile centuncularis (Linnaeus, 1758), Hylaeus annularis (Kirby, 1802), Nomada flavopicta (Kirby, 1802), Colletes similis Schenck, 1853, Hylaeus sinuatus (Schenck, 1853), Halictus rubicundus (Christ, 1791), Megachile albisecta (Klug in Germar, 1817), Lasioglossum pygmaeum (Schenck, 1853), Nomada furva Panzer, 1798, Melitta tricincta Kirby, 1802, Osmia melanogaster Spinola, 1807, Bombus hypnorum (Linnaeus, 1758), Panurgus dentipes Latreille, 1811, Osmia aurulenta (Panzer, 1799), Halictus patellatus Morawitz, 1873, Sphecodes crassus Thomson, 1870, Sphecodes marginatus Hagens, 1882, Lasioglossum subhirtum (Lepeletier, 1841), Sphecodes gibbus (Linnaeus, 1758), Stelis annulata (Lepeletier, 1841), Megachile melanopyga Costa, 1863, Andrena minutuloides Perkins, 1914, Lasioglossum majus (Nylander, 1852), Lasioglossum minutissimum (Kirby, 1802), Tetralonia malvae (Rossi, 1790), Andrena nana (Kirby, 1802), Andrena hedikae Jaeger, 1934, Andrena trimmerana (Kirby, 1802), Andrena angustior (Kirby, 1802), Anthophora plumipes (Pallas, 1772), Andrena dorsata (Kirby, 1802), Andrena nitida (Muller, 1776), Andrena haemorrhoa (Fabricius, 1781), Osmia bicornis (Linnaeus, 1758), Andrena lagopus Latreille, 1809, Halictus tetrazonius (Klug in Germar, 1817), Andrena distinguenda Schenck, 1871, Andrena pusilla Perez, 1903, Andrena livens Perez, 1895, Andrena gravida Imhoff, 1832, Andrena nigroaenea (Kirby, 1802), Lasioglossum pallens (Brulle, 1832), Andrena fulvata Stoeckhert, 1930, Andrena cineraria (Linnaeus, 1758), Colletes cunicularius (Linnaeus, 1761), Lasioglossum laevigatum (Kirby, 1802), Lasioglossum pauperatum (Brulle, 1832), Andrena combinata (Christ, 1791), Andrena helvola (Linnaeus, 1758), Andrena ferox Smith, 1847, Andrena pallitarsis Perez, 1903, Lasioglossum xanthopus (Kirby, 1802), Lasioglossum crassepunctatum (Bluthgen, 1923), Andrena nigroolivacea Dours, 1873, Lasioglossum nitidulum (Fabricius, 1804), Andrena bucephala Stephens, 1846, Osmia bicolor (Schrank, 1781), Anthophora plagiata (Illiger, 1806), Osmia cornuta (Latreille, 1805), Bombus sylvestris (Lepeletier, 1833), Andrena scotica Perkins, 1916, Lasioglossum truncaticolle (Morawitz, 1877), Andrena strohmella Stoeckhert, 1928, Andrena nitidula Perez, 1903, Andrena rufula Schmiedeknecht, 1883, Andrena fulvago (Christ, 1791), Andrena bicolorata (Rossi, 1790), Andrena pilipes Fabricius, 1781, Andrena bimaculata (Kirby, 1802), Andrena humilis Imhoff, 1832, Lasioglossum griseolum (Morawitz, 1872), Hoplitis adunca (Panzer, 1798), Lasioglossum tricinctum (Schenck, 1874), Andrena griseobalteata Dours, 1872, Melecta albifrons (Forster, 1771), Andrena russula Lepeletier de Saint-Fargeau, 1841, Andrena flavilabris Schenck, 1874, Chelostoma florisomne (Linnaeus, 1758), Andrena leucolippa Perez, 1895, Lasioglossum costulatum (Kriechbaumer, 1873), Eucera interrupta Bar, 1850, Sphecodes ruficrus (Erichson in Waltl, 1835), Chelostoma emarginatum (Nylander, 1856), Lasioglossum mesosclerum (Perez, 1903), Eucera numida Lepeletier, 1841, Seladonia confusa (Smith, 1853), Andrena impressa Warncke, 1967, Melecta italica Radoszkowski, 1876, Andrena rhenana Stoeckhert, 1930, Hylaeus cornutus Curtis, 1831, Halictus fulvipes (Klug in Germar, 1817), Andrena chrysopyga Schenck, 1853, Osmia brevicornis (Fabricius, 1798), Bombus muscorum (Linnaeus, 1758), Lasioglossum angusticeps (Perkins, 1895), Andrena simontornyella Noskiewicz, 1939, Andrena lathyri Alfken, 1899, Lasioglossum medinai (Vachal, 1895), Osmia niveata (Fabricius, 1804), Andrena viridescens Viereck, 1916, Andrena labiata Fabricius, 1781, Andrena vetula Lepeletier, 1841, Nomada striata Fabricius, 1793, Nomada goodeniana (Kirby, 1802), Bombus pomorum (Panzer, 1802), Andrena subopaca Nylander, 1848, Nomada fabriciana (Linnaeus, 1767), Nomada sexfasciata Panzer, 1799, Nomada bifasciata Olivier, 1811, Nomada melathoracica Imhoff, 1834, Sphecodes pseudofasciatus Bluthgen, 1925, Nomada zonata Panzer, 1797, Nomada lathburiana (Kirby, 1802), Andrena fulva (Muller, 1766), Andrena mitis Schmiedeknecht, 1883, Nomada flava Panzer, 1797, Lasioglossum bluethgeni Ebmer, 1971, Andrena thoracica (Fabricius, 1775), Rhodanthidium septemdentatum (Latreille, 1809), Stelis breviuscula (Nylander, 1848), Sphecodes majalis Perez, 1903, Osmia leaiana (Kirby, 1802), Nomada ruficornis (Linnaeus, 1758), Anthophora retusa (Linnaeus, 1758), Nomada tridentirostris Dours, 1873, Panurgus calcaratus (Scopoli, 1763), Nomada leucophthalma (Kirby, 1802), Andrena colletiformis Morawitz, 1873, Nomada panzeri Lepeletier, 1841, Nomada succincta Panzer, 1798, Megachile ligniseca (Kirby, 1802), Andrena hattorfiana (Fabricius, 1775), Hylaeus rubicola Saunders, 1850, Nomada villosa Thomson, 1870, Sphecodes rubicundus Hagens, 1875, Andrena synadelpha Perkins, 1914, Nomada hirtipes Perez, 1884, Nomada bluethgeni Stockhert, 1944
Subspecies: Megachile argentata argentata (Fabricius, 1793), Bombus terrestris terrestris (Linnaeus, 1758), Bombus terrestris lusitanicus Kruger, 1956, Bombus pascuorum maculatus Vogt, 1909, Anthophora retusa retusa (Linnaeus, 1758), Lasioglossum discus discus (Smith, 1853), Andrena angustior impressa Warncke, 1967

Spatial coverage

General spatial coverage: The Apoidea specimens come from south-west France, in the LTSER Vallées et Coteaux de Gascogne embedded in the ZA Pygar (Ouin et al. 2021).
Coordinates: 43°8'24''N and 43°35'24''N Latitude; 0°29'24''E and 1°7'12''E Longitude 

Temporal coverage: May 26, 2010 - April 12, 2022

Natural collections description

Collection name: DYNAFOR Lab (UMR 1201 INRAE, INP-ENSAT, EI PURPAN), located at INP-ENSAT, Av. de l'Agrobiopole, 31326 Auzeville-Tolosane, FRANCE
Specimen preservation method: Pinned
Specimen preservation method: Dried

Methods

Method step description: See Sampling description and Quality control
Study extent description: The Apoidea specimens come from south-west France, in the LTSER Vallées et Coteaux de Gascogne embedded in the ZA Pygar. 22,505 specimens were collected in the French department Haute-Garonne and 1,824 in the department Gers.

Data range: 2010-7-02 - 2022-4-12.
Sampling is inconsistent over time, depending on the projects being carried each year and the protocols chosen. The number of sampling events varies from 10 to 452 between 2010 and 2022, with an average of 127 samplings per year over this period.
Sampling description: Wild bees were sampled in various habitat types (cultivated and semi-natural, Figure 1) including field margins of crops and inner crops (12,701 individuals), small forests and their edges (7,856 individuals), hedgerows (119 individuals) and permanent grasslands (294 individuals). The precision of the GPS coordinates given in the dataset has been reduced to preserve the locality of vulnerable species. Depending on the project, two sampling methods were implemented : 1) insect net catching along transects for 3,888 individuals belonging to 183 species (described in Rivers-Moore et al. 2020), or 2) one to two sets of three to six coloured pan traps per sampling site (blue, yellow and white UV colour paint) (e.g. Rivers-Moore et al. 2023) for 20,441 individuals belonging to 203 species.
Quality control description: Before 2015, depending on the project and on the people involved, the most obvious and common species were identified by Samantha Bailley, Romain Carrié, Clara Singh and Léa Frontero, all the others were sent to experts (see below), except for BioBio project for which all specimens were sent to David Genoud.

After 2015, all sampled specimens were sorted out to order and family ranks and pinned, before being sent to national experts of Apoidea apiformes group : Rémi Rudelle, David Genoud, Eric Dufrêne, Alain Pauly and Pierre Rasmont.

For four species complexes ( Bombus gr. Terrestris , Halictus gr. Simplex , Seladonia gr. Smaragdula and Hylaeus gr. brevicornis ), the taxonomy is not stabilized for the female individuals, and the current identification tools (dichotomous keys) do not allow for a clear separation of the species within these complexes. Taxonomists agree to use the name of the complex for specimen identification when it could be one of the following species: Bombus terrestris , Bombus lucorum , Bombus magnus , and Bombus cryptarum (4 species of the Bombus gr. terrestris ), Halictus simplex , Halictus eurygnathus , Halictus langobardicus , Halictus crenicornis , Halictus tridivisus , Halictus adjikenticus , Halictus carinthiacus , Halictus gruenwaldti , and Halictus pyrenaeus (9 species of the Halictus gr. simplex ), Seladonia smaragdula , Seladonia gemmella and Seladonia submediterranea (3 species of the Seladonia gr. Smaragdula , Pauly et al. 2015), or one of the 22 species from Hylaeus gr. Brevicornis (Dathe et al. 2022). In the present dataset, the specimens belonging to these groups have been identified either to the species level (when morphological characters allowed) or to its complex species group (for female individuals). Ongoing barcoding initiatives may help to clarify the reliable morphological characteristics for identifying species within these complexes.

Recently, a fraction of the specimens contained in the collection (534 specimens) and covering 60% of wild bee diversity sampled in ZA-PYGAR has been used to generate DNA barcoding reference sequences, allowing a cross-validation of the specimen identification. For each specimen chosen, one front leg was sampled and used for further molecular processes involving DNA extraction, marker amplification and sequencing (articles in prep., Hebert et al 2003, Folmer et al 1994, Clarke et al 2014). Because of the existence of groups of species particularly recalcitrant to sequencing of the standard CO1 marker, two complementary markers were targeted: CO1 and 16S (Sheffield et al 2017, Villalta et al 2021). A total of 110 and 347 specimens were sampled to provide CO1 marker barcodes and 16S marker barcodes, respectively, while 76 specimens were used to generate reference barcodes for both markers. DNA barcoding results are currently being analysed and discussed with experts. In case of incongruence between morphological and molecular identifications, the specimens are subjected to re-observation of morphological characters. This process is under progress. 

Datasets

Dataset description
Object name: Darwin Core Archive Wild bees (Apoidea: Anthophila) of South-West France: more than 10 years of inventories in mosaic landscapes of ZA-PYGAR
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: http://dynids.toulouse.inra.fr:8180/ipt/archive.do?r=baseabeilles
Publication date of data: 2024-12-05
Language: English
Licences of use: Creative Commons Attribution (CC-BY) 4.0 License

Metadata language: English
Date of metadata creation: 2024-07-17
Hierarchy level: Dataset

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